Cette biodégradation est basée sur le fonctionnement d’un consortium bactérien complexe, composé de bactéries autotrophes et hétérotrophes, associées sous. Request PDF on ResearchGate | Influence possible des protozoaires sur le taux de mortalité des bactéries autotrophes nitrifiantes | Le modele de l’IAWQ du. Bactérie (Bacteria): Les bactéries sont des organismes vivants unicellulaires, très Les bactéries autotrophes telles que Nitrobacter, active dans les processus.

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Different methods of measuring the nitrifying activity exist. These experiments consisted in measuring the effect of different combinations of N-serve, ethanol the organic solvent of N-serve and chlorate, on N-oxidation rates and C incorporation rates on samples of the two nitrifying enrichment cultures ammonium- and nitrite-oxidising bacteria.

The inhibitors effects were also determined on the C incorporation rates of heterotrophic bacteria. The measurement of the activity and biomass of nitrifying bacteria is thus essential to understand and quantify the general nitrogen fluxes in those environments.

The general validity of the method was demonstrated by experiments on nitrification inhibitors in enrichment cultures. Bacteria which are able to multiply by using inorganic matter as the only source of carbon and nitrogen. Bicarbonate was measured by acid titration in natural water samples, and with the evolution method for culture samples. However, the ethanolic solution of N-serve had an unwanted result on C incorporation.

In which subject field? Our conclusion is that the results presented in this paper allow the validation of the 14C-bicarbonate incorporation method with and without inhibitors to measure the nitrifying activity.

At the present time, the most used methods are dealing with isotopic tracers: By regenerating oxidised forms of nitrogen nitratethe nitrification process plays an important role in the nitrogen cycle of aquatic environments. Switch to classic wutotrophe. This potential activity can be used to estimate the nitrifying biomass baterie considering a conversion factor of 0. A good alternative to these methods consists in the measurement of potential nitrifying activity which is correlated to the nitrifying biomass.


This factors allowed us to establish a relationship between potential nitrifying activity measurements and nitrifying biomass: Several methods were used for our study: In this case, carbon incorporation rates can be converted in ammonium oxidation rates with the use of the conversion factor 0. C incorporation rates are measured by the incubation of samples with 14C-bicarbonate, the samples being filtered on 0. Writing tools A collection of writing tools that cover the many facets of English and French grammar, style and usage.

The most frequently used enumeration methods of nitrifying bacteria are not very satisfactory. The organic solvent enhanced heterotrophic incorporation of C which totally masked out the autotrophic contribution of nitrifying bacteria.

Pure cultures of Nitrosomonas europaea and Nitrobacter winogradskyi were obtained from the National Collection of Industrial and Marine Bacteria Aberdeen, Scotland and a continuous enrichment culture of mixed heterotrophic bacteria, without nitrifying organisms, was obtained with a freshwater inoculum by imposing a residence time of 2 hours less than the generation time of nitrifying bacteria.

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Recently developed gene-probes techniques work well for the identification of particular strains, but are not yet useful for the numeration.

Change the order of display of the official languages of Canada English first French first Option to display the non-official languages Spanish or Portuguese Lew Spanish Portuguese Display definitions, contexts, etc.

A collection of writing tools that cover the many facets of English and French grammar, style and usage. Ammonium was measured with the indophenol blue method, nitrate was reduced in nitrite on a cadmium bed and nitrite was measured with the sulfanilamide method.


Nitrifying activity, nitrifying biomass, 14C-bicarbonate incorporation, nitrification inhibitors, N-serve, chlorate. Biovolumes were estimated according to cell size and autotrkphe in biomasses according to a conversion factor determined experimentally with a carbon analyser. Revue des sciences de l’eau 11, no. N-serve, allyl thio-urea, acetylene, methylfluoride and dimethyl ether, as most used.

Une réévaluation de la methode d’incorporation – Revue des sciences de l’eau – Érudit

To measure potential nitrifying activities, the optimal growth conditions of nitrifying bacteria were determined on enrichment cultures: Access a collection of Canadian resources on all aspects of English and French, including quizzes. Revue des sciences de l’eau11 2— The first methods developed were based on the use of specific nitrification inhibitors: For this reason N-serve was added in the empty flask before the sample to allow the evaporation of the solvent.

FAQ Frequently asked questions Display options. Classical culture techniques most probable number and immunofluorescence techniques are known to greatly underestimate the numbers of active organisms.

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By acting this way, inhibition of autotrophic C incorporation by nitrifying bacteria was also complete, while heterotrophic incorporation was unaffected. The method can be used in in situ conditions, to allow real nitrifying activities measurements in samples. The main differences of our protocol to the original ones is that we propose the use of a combination of 2 inhibitors, N-serve and chlorate, and the elimination by evaporation of the organic solvent of N-serve ethanol to avoid any interference with the heterotrophic populations.